It is helpful if you provide any relevant information about the software and Giotto object you are using. See below for some tips to get this information.
What version of R are you using?
R.version.string"R version 4.0.4 (2021-02-15)"What version of Giotto are you using?
packageVersion("Giotto")2.0.0.9044Are you using the Giotto environment or your own python path?
# Check Giotto environment
Giotto:::checkGiottoEnvironment()giotto environment found at
/Users/natalie_dr/Library/r-miniconda/envs/giotto_env/bin/pythonw Show sequential processing steps/commands in the Giotto object
Giotto:::showProcessingSteps(my_giotto_object)Processing steps:
0_normalize Show the structure of the Giotto object
str(my_giotto_object, max.level = 3)Formal class 'giotto' [package "Giotto"] with 20 slots
..@ expression :List of 1
.. ..$ rna:List of 3
..@ expression_feat : chr "rna"
..@ spatial_locs :List of 1
.. ..$ raw:Classes ‘data.table’ and 'data.frame': 73655 obs. of 4 variables:
.. .. ..- attr(*, ".internal.selfref")=<externalptr>
..@ spatial_info : NULL
..@ cell_metadata :List of 1
.. ..$ rna:Classes ‘data.table’ and 'data.frame': 73655 obs. of 1 variable:
.. .. ..- attr(*, ".internal.selfref")=<externalptr>
..@ feat_metadata :List of 1
.. ..$ rna:Classes ‘data.table’ and 'data.frame': 161 obs. of 1 variable:
.. .. ..- attr(*, ".internal.selfref")=<externalptr>
..@ feat_info : NULL
..@ cell_ID : chr [1:73655] "cell_1" "cell_2" "cell_3" "cell_4" ...
..@ feat_ID :List of 1
.. ..$ rna: chr [1:161] "Ace2" "Adora2a" "Aldh1l1" "Amigo2" ...
..@ spatial_network : NULL
..@ spatial_grid : NULL
..@ spatial_enrichment : NULL
..@ dimension_reduction: NULL
..@ nn_network : NULL
..@ images : NULL
..@ parameters :List of 1
.. ..$ 0_normalize: Named chr [1:13] "my_giotto_object" "standard" "TRUE" "6000" ...
.. .. ..- attr(*, "names")= chr [1:13] "gobject" "norm_methods" "library_size_norm" "scalefactor" ...
..@ instructions :List of 11
.. ..$ python_path: chr "/Users/natalie_dr/Library/r-miniconda/envs/giotto_env/bin/pythonw"
.. ..$ show_plot : logi TRUE
.. ..$ return_plot: logi TRUE
.. ..$ save_plot : logi FALSE
.. ..$ save_dir : chr "/Users/natalie_dr/Desktop/my_giotto/giotto_user_help"
.. ..$ plot_format: chr "png"
.. ..$ dpi : num 300
.. ..$ units : chr "in"
.. ..$ height : num 9
.. ..$ width : num 9
.. ..$ is_docker : logi FALSE
..@ offset_file : NULL
..@ OS_platform : chr "unix"
..@ join_info : NULL
my_giotto_object <- createGiottoObject(raw_exprs = "path/to/expression/data",
spatial_locs = "path/to/spatial/data")Warning message:
In createGiottoObject(raw_exprs = "/Users/username/path/to/data", :
raw_exprs argument is deprecated, use expression argument in the future Solution: The code has still run and your object has been created, but in the future use the following command:
my_giotto_object <- createGiottoObject(expression = "path/to/expression/data",
spatial_locs = "path/to/spatial/data")Error in adjustGiottoMatrix(gobject = my_giotto_object, expression_values = c("normalized"): covariate column name(s) were not found in the cell metadata Solution: In this case, you would need to check your cell
metadata (my_giotto_object@cell_metadata) and make
appropriate adjustments.
Error in methods::as(as.matrix(x[, -1]), "Matrix") :
no method or default for coercing "matrix" to "Matrix"Solution: Ensure that the Matrix library is loaded:
Some steps before posting an issue:
Google (or whichever search engine you prefer) the error
Look up the documentation for the package the error came from
What code/data did you use? (copy-pasted or an example is helpful)
What did you expect your result to be?
What was your result?
For us to be able to help you at the best of our ability, it’s helpful if you can provide a reproducible example. This just means that anyone who reads your GitHub issue should be able to accurately reproduce your error.
For more information on minimal reproducible examples check out this link.
And to see what other people recommend, please check out this forum.
Please use the GitHub “insert code” function or wrap your code like so:
`x <- “hello world”
print(x)`
If applicable, provide a screenshot of the issue
I am having trouble adjusting my Giotto matix. I have inputted the following code:
my_giotto_object <- adjustGiottoMatrix(gobject = my_giotto_object,
expression_values = c('normalized'),
covariate_columns = c('nr_genes',
'total_expr'))I expected that my matrix would be appropriately adjusted, but I got the following error:
Error in adjustGiottoMatrix(gobject = my_giotto_object, expression_values = c("normalized"): covariate column name(s) were not found in the cell metadata For more context, I am running R version 4.04 and Giotto version 2.0.0.9044. I can send you the data I used if necessary.